CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli

Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We used Cas9‐mediated recombineering to introduce a library of mut...

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Detalles Bibliográficos
Autores principales: Choudhury, Alaksh, Fenster, Jacob A, Fankhauser, Reilly G, Kaar, Joel L, Tenaillon, Olivier, Gill, Ryan T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073797/
https://www.ncbi.nlm.nih.gov/pubmed/32175691
http://dx.doi.org/10.15252/msb.20199265

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073797/
https://www.ncbi.nlm.nih.gov/pubmed/32175691
http://dx.doi.org/10.15252/msb.20199265

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