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CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli
Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We used Cas9‐mediated recombineering to introduce a library of mut...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073797/ https://www.ncbi.nlm.nih.gov/pubmed/32175691 http://dx.doi.org/10.15252/msb.20199265 |