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Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse

Campylobacter spp. are among the microorganisms most commonly associated with foodborne disease. Swine are known to be the main reservoir of Campylobacter coli and a possible source infection of humans as a result of carcass contamination at slaughter. The aim of this study was to evaluate the preva...

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Autores principales: Di Donato, Guido, Marotta, Francesca, Nuvoloni, Roberta, Zilli, Katiuscia, Neri, Diana, Di Sabatino, Daria, Calistri, Paolo, Di Giannatale, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074678/
https://www.ncbi.nlm.nih.gov/pubmed/32046038
http://dx.doi.org/10.3390/microorganisms8020222
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author Di Donato, Guido
Marotta, Francesca
Nuvoloni, Roberta
Zilli, Katiuscia
Neri, Diana
Di Sabatino, Daria
Calistri, Paolo
Di Giannatale, Elisabetta
author_facet Di Donato, Guido
Marotta, Francesca
Nuvoloni, Roberta
Zilli, Katiuscia
Neri, Diana
Di Sabatino, Daria
Calistri, Paolo
Di Giannatale, Elisabetta
author_sort Di Donato, Guido
collection PubMed
description Campylobacter spp. are among the microorganisms most commonly associated with foodborne disease. Swine are known to be the main reservoir of Campylobacter coli and a possible source infection of humans as a result of carcass contamination at slaughter. The aim of this study was to evaluate the prevalence of C. coli contamination in swine carcasses, the antimicrobial resistance (AMR) patterns of isolates and the genetic diversity between strains obtained from swine and those isolated from humans. The prevalence of contamination was higher on carcasses (50.4%) than in faeces (32.9%). The 162 C. coli isolated from swine were examined by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The results of PFGE indicated a high genetic diversity among the isolates, with 25 different PFGE types. MLST assigned 51 sequence types (STs) to isolates. The most common genotype was ST-854 (16.04%), ST-9264 (10.49 %) and ST-1016 (6.08 %). Results of AMR showed a high resistance to quinolones and fluoroquinolones together with aminoglycosides and tetracycline. Many strains were multi-resistant with predominant R-type TeSCipNa (57%). Five resistance genes were detected along with mutation in the gyrA gene. A strong correlation between phenotypic and genotypic resistance was found for fluoroquinolone and tetracycline. Genetic profiles obtained in swine isolates were compared to those of 11 human strains. All human strains and 64.19% of animal strains (104/162) were assigned to the ST-828 clonal complex.
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spelling pubmed-70746782020-03-20 Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse Di Donato, Guido Marotta, Francesca Nuvoloni, Roberta Zilli, Katiuscia Neri, Diana Di Sabatino, Daria Calistri, Paolo Di Giannatale, Elisabetta Microorganisms Article Campylobacter spp. are among the microorganisms most commonly associated with foodborne disease. Swine are known to be the main reservoir of Campylobacter coli and a possible source infection of humans as a result of carcass contamination at slaughter. The aim of this study was to evaluate the prevalence of C. coli contamination in swine carcasses, the antimicrobial resistance (AMR) patterns of isolates and the genetic diversity between strains obtained from swine and those isolated from humans. The prevalence of contamination was higher on carcasses (50.4%) than in faeces (32.9%). The 162 C. coli isolated from swine were examined by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The results of PFGE indicated a high genetic diversity among the isolates, with 25 different PFGE types. MLST assigned 51 sequence types (STs) to isolates. The most common genotype was ST-854 (16.04%), ST-9264 (10.49 %) and ST-1016 (6.08 %). Results of AMR showed a high resistance to quinolones and fluoroquinolones together with aminoglycosides and tetracycline. Many strains were multi-resistant with predominant R-type TeSCipNa (57%). Five resistance genes were detected along with mutation in the gyrA gene. A strong correlation between phenotypic and genotypic resistance was found for fluoroquinolone and tetracycline. Genetic profiles obtained in swine isolates were compared to those of 11 human strains. All human strains and 64.19% of animal strains (104/162) were assigned to the ST-828 clonal complex. MDPI 2020-02-07 /pmc/articles/PMC7074678/ /pubmed/32046038 http://dx.doi.org/10.3390/microorganisms8020222 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Donato, Guido
Marotta, Francesca
Nuvoloni, Roberta
Zilli, Katiuscia
Neri, Diana
Di Sabatino, Daria
Calistri, Paolo
Di Giannatale, Elisabetta
Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse
title Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse
title_full Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse
title_fullStr Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse
title_full_unstemmed Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse
title_short Prevalence, Population Diversity and Antimicrobial Resistance of Campylobacter coli Isolated in Italian Swine at Slaughterhouse
title_sort prevalence, population diversity and antimicrobial resistance of campylobacter coli isolated in italian swine at slaughterhouse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074678/
https://www.ncbi.nlm.nih.gov/pubmed/32046038
http://dx.doi.org/10.3390/microorganisms8020222
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