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Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity

“Antibody-breeding” has provided therapeutic/diagnostic antibody mutants with greater performance than native antibodies. Typically, random point mutations are introduced into the V(H) and V(L) domains of parent antibodies to generate diverse libraries of single-chain Fv fragments (scFvs), from whic...

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Detalles Bibliográficos
Autores principales:	Oyama, Hiroyuki, Kiguchi, Yuki, Morita, Izumi, Yamamoto, Chika, Higashi, Yuka, Taguchi, Miku, Tagawa, Tatsuya, Enami, Yuri, Takamine, Yuriko, Hasegawa, Hanako, Takeuchi, Atsuko, Kobayashi, Norihiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075871/ https://www.ncbi.nlm.nih.gov/pubmed/32179767 http://dx.doi.org/10.1038/s41598-020-61529-7

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