Practical Guide for Quantification of In Vivo Degradation Rates for Therapeutic Proteins with Single-Cell Resolution Using Fluorescence Ratio Imaging

Many tools for studying the pharmacokinetics of biologics lack single-cell resolution to quantify the heterogeneous tissue distribution and subsequent therapeutic degradation in vivo. This protocol describes a dual-labeling technique using two near-infrared dyes with widely differing residualization...

Descripción completa

Detalles Bibliográficos
Autores principales: Nessler, Ian, Cilliers, Cornelius, Thurber, Greg M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076450/
https://www.ncbi.nlm.nih.gov/pubmed/32033318
http://dx.doi.org/10.3390/pharmaceutics12020132

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076450/
https://www.ncbi.nlm.nih.gov/pubmed/32033318
http://dx.doi.org/10.3390/pharmaceutics12020132

Ejemplares similares