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Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real-Time PCR and RT-qPCR Diagnostics in Virology

Real-time molecular techniques have become the reference methods for direct diagnosis of pathogens. The reduction of steps is a key factor in order to decrease the risk of human errors resulting in invalid series and delayed results. We describe here a process of preparation of oligonucleotide prime...

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Detalles Bibliográficos
Autores principales: Thirion, Laurence, Dubot-Peres, Audrey, Pezzi, Laura, Corcostegui, Iban, Touinssi, Mhammed, de Lamballerie, Xavier, Charrel, Remi N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077261/
https://www.ncbi.nlm.nih.gov/pubmed/32019076
http://dx.doi.org/10.3390/v12020159