RNA-guided retargeting of Sleeping Beauty transposition in human cells

An ideal tool for gene therapy would enable efficient gene integration at predetermined sites in the human genome. Here we demonstrate biased genome-wide integration of the Sleeping Beauty (SB) transposon by combining it with components of the CRISPR/Cas9 system. We provide proof-of-concept that it...

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Detalles Bibliográficos
Autores principales: Kovač, Adrian, Miskey, Csaba, Menzel, Michael, Grueso, Esther, Gogol-Döring, Andreas, Ivics, Zoltán
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077980/
https://www.ncbi.nlm.nih.gov/pubmed/32142408
http://dx.doi.org/10.7554/eLife.53868

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7077980/
https://www.ncbi.nlm.nih.gov/pubmed/32142408
http://dx.doi.org/10.7554/eLife.53868

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