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Use of chimeric influenza viruses as a novel internal control for diagnostic rRT-PCR assays

Real-time quantitative reverse transcriptase polymerase chain reaction (rRT-PCR) is now widely used to detect viral pathogens in various human specimens. The application of internal controls to validate the entire process of these assays is necessary to prevent false-negative results caused by unexp...

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Detalles Bibliográficos
Autores principales: Wang, Xueliang, Liu, Fen, Jiang, Lingli, Bao, Yun, Xiao, Yanqun, Wang, Hualiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080162/
https://www.ncbi.nlm.nih.gov/pubmed/26474983
http://dx.doi.org/10.1007/s00253-015-7042-y