Cargando…

Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry

[Image: see text] Size-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a useful tool to study proteins in their native state. However, whether the applied eluent conditions actually pre...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ventouri, Iro K., Malheiro, Daniel B. A., Voeten, Robert L. C., Kok, Sander, Honing, Maarten, Somsen, Govert W., Haselberg, Rob
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2020
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081181/ https://www.ncbi.nlm.nih.gov/pubmed/32107919 http://dx.doi.org/10.1021/acs.analchem.9b04961

Ejemplares similares

Capillary Electrophoresis: Trends and Recent Advances
por: Voeten, Robert L. C., et al.
Publicado: (2018)

Characterizing Non-covalent Protein Complexes Using Asymmetrical Flow Field-Flow Fractionation On-Line Coupled to Native Mass Spectrometry
por: Ventouri, Iro Konstantina, et al.
Publicado: (2023)

Probing Polyester Branching by Hybrid Trapped Ion-Mobility Spectrometry–Tandem Mass Spectrometry
por: Voeten, Robert L. C., et al.
Publicado: (2021)

Affinity profiling of monoclonal antibody and antibody-drug-conjugate preparations by coupled liquid chromatography-surface plasmon resonance biosensing
por: Lakayan, Dina, et al.
Publicado: (2018)

Rapid characterization of biotherapeutic proteins by size-exclusion chromatography coupled to native mass spectrometry
por: Haberger, Markus, et al.
Publicado: (2015)

Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins
por: Wouters, Sam, et al.
Publicado: (2021)

Capillary electrophoresis-mass spectrometry using noncovalently coated capillaries for the analysis of biopharmaceuticals
por: Haselberg, R., et al.
Publicado: (2011)

THE EQUILIBRIA BETWEEN NATIVE AND DENATURED HEMOGLOBIN IN SALICYLATE SOLUTIONS AND THE THEORETICAL CONSEQUENCES OF THE EQUILIBRIUM BETWEEN NATIVE AND DENATURED PROTEIN
por: Anson, M. L., et al.
Publicado: (1934)

Quality of Original and Biosimilar Epoetin Products
por: Brinks, Vera, et al.
Publicado: (2010)

Mass spectrometry data and size exclusion chromatography profiles of Australian taipan venom toxins
por: Harrison, Julian A., et al.
Publicado: (2016)

Strategies in size-exclusion chromatography /
Publicado: (1996)

Liquid Core Waveguide Cell with In Situ Absorbance Spectroscopy and Coupled to Liquid Chromatography for Studying Light-Induced Degradation
por: Groeneveld, Iris, et al.
Publicado: (2022)

Direct coupling of size exclusion chromatography and mass spectrometry for the characterization of complex monoclonal antibody products
por: Murisier, Amarande, et al.
Publicado: (2022)

IMMUNOCHEMICAL PROPERTIES OF NATIVE AND DENATURED HORSE SERUM GLOBULINS
por: Erickson, John O., et al.
Publicado: (1945)

AN IMMUNOLOGICAL STUDY OF NATIVE, DENATURED, AND REVERSED SERUM ALBUMIN
por: Miller, Benjamin F.
Publicado: (1933)

Liposome retention in size exclusion chromatography
por: Ruysschaert, Tristan, et al.
Publicado: (2005)

Chapter 5 Size-exclusion chromatography
por: Silberring, J., et al.
Publicado: (2004)

Native size-exclusion chromatography-mass spectrometry: suitability for antibody–drug conjugate drug-to-antibody ratio quantitation across a range of chemotypes and drug-loading levels
por: Jones, Jay, et al.
Publicado: (2019)

Native Size-Exclusion Chromatography–Based Mass Spectrometry Reveals New Components of the Early Heat Shock Protein 90 Inhibition Response Among Limited Global Changes
por: Samant, Rahul S., et al.
Publicado: (2022)

Native Liquid Chromatography and Mass Spectrometry to Structurally and Functionally Characterize Endo-Xylanase Proteoforms
por: van Schaick, Guusje, et al.
Publicado: (2022)

THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN
por: Anson, M. L., et al.
Publicado: (1933)

THE EQUILIBRIUM BETWEEN ACTIVE NATIVE TRYPSIN AND INACTIVE DENATURED TRYPSIN
por: Anson, M. L., et al.
Publicado: (1934)

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase
por: Zaroog, Mohammed Suleiman, et al.
Publicado: (2013)

The denatured state of HIV‐1 protease under native conditions
por: Rösner, Heike I., et al.
Publicado: (2021)

Comparative Analysis of Antibodies and Heavily Glycosylated Macromolecular Immune Complexes by Size-Exclusion Chromatography Multi-Angle Light Scattering, Native Charge Detection Mass Spectrometry, and Mass Photometry
por: den Boer, Maurits A., et al.
Publicado: (2021)

A Deadenylase Assay by Size-Exclusion Chromatography
por: He, Guang-Jun, et al.
Publicado: (2012)

Analytical Size Exclusion Chromatography Coupled with Mass Spectrometry in Parallel with High-Throughput Venomics and Bioassaying for Venom Profiling
por: Terzioglu, Sedef, et al.
Publicado: (2023)

Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography
por: Park, Seong-Jun, et al.
Publicado: (2015)

Probing Protein Glycation by Chromatography and Mass Spectrometry: Analysis of Glycation Adducts
por: Soboleva, Alena, et al.
Publicado: (2017)

Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size Exclusion Chromatography for Proteome-wide Cross-Linking Mass Spectrometry
por: Sinn, Ludwig R., et al.
Publicado: (2022)

Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry
por: Tengattini, Sara, et al.
Publicado: (2016)

Size-exclusion chromatography (SEC) of branched polymers and polysaccharides
por: Gaborieau, Marianne, et al.
Publicado: (2010)

Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and their Aggregates
por: Hong, Paula, et al.
Publicado: (2012)

Retention Mechanism of Branched Macromolecules in Size Exclusion Chromatography
por: Netopilík, Miloš, et al.
Publicado: (2020)

Purification of turkey coronavirus by Sephacryl size-exclusion chromatography
por: Loa, C.C, et al.
Publicado: (2002)

Limited Lactosylation of Beta-Lactoglobulin from Cow’s Milk Exerts Strong Influence on Antigenicity and Degranulation of Mast Cells
por: Bosman, Gerlof P., et al.
Publicado: (2021)

Enantioselective micellar electrokinetic chromatography of dl‐amino acids using (+)‐1‐(9‐fluorenyl)‐ethyl chloroformate derivatization and UV‐induced fluorescence detection
por: Prior, Amir, et al.
Publicado: (2018)

Recent applications of chemometrics in one‐ and two‐dimensional chromatography
por: Bos, Tijmen S., et al.
Publicado: (2020)

High Performance Size Exclusion Chromatography-Inductively Coupled Plasma-Mass Spectrometry to Study the Copper and Cadmium Complexation with Humic Acids
por: Radaelli, Marta, et al.
Publicado: (2019)

Modeling Formamide Denaturation of Probe-Target Hybrids for Improved Microarray Probe Design in Microbial Diagnostics
por: Yilmaz, L. Safak, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_c16q116ksdjd295jegbmmpusdk