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Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis
[Image: see text] The pH dependence of the trypsin-catalyzed hydrolysis of N-α-benzyloxycarbonyl-l-lysine p-nitroanilide has been studied at 25 °C. k(cat)/K(M) was maximal at alkaline pH values but decreased with decreasing pH. k(cat)/K(M) was dependent on free enzyme pK(a) values of 6.75 ± 0.09 and...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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American Chemical Society
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081292/ https://www.ncbi.nlm.nih.gov/pubmed/32201777 http://dx.doi.org/10.1021/acsomega.9b03750 |