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Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM
Fluorescence lifetime imaging (FLIM) is a quantitative, intensity-independent microscopical method for measurement of diverse biochemical and physical properties in cell biology. It is a highly effective method for measurements of Förster resonance energy transfer (FRET), and for quantification of p...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083966/ https://www.ncbi.nlm.nih.gov/pubmed/32198437 http://dx.doi.org/10.1038/s41598-020-61478-1 |