Intra-Molecular Homologous Recombination of Scarless Plasmid

Although many methods have been reported, plasmid construction compromises transformant efficiency (number of transformants per ng of DNAs) with plasmid accuracy (rate of scarless plasmids). An efficient method is two-step PCR serving DNA amplification. An accurate method is ExnaseII cloning serving...

Descripción completa

Detalles Bibliográficos
Autores principales: Liang, Yaping, Zhang, Yu, Liu, Liangwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084384/
https://www.ncbi.nlm.nih.gov/pubmed/32131382
http://dx.doi.org/10.3390/ijms21051697

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084384/
https://www.ncbi.nlm.nih.gov/pubmed/32131382
http://dx.doi.org/10.3390/ijms21051697

Ejemplares similares