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Styrene maleic-acid lipid particles (SMALPs) into detergent or amphipols: An exchange protocol for membrane protein characterisation

Membrane proteins are traditionally extracted and purified in detergent for biochemical and structural characterisation. This process is often costly and laborious, and the stripping away of potentially stabilising lipids from the membrane protein of interest can have detrimental effects on protein...

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Detalles Bibliográficos
Autores principales: Hesketh, Sophie J., Klebl, David P., Higgins, Anna J., Thomsen, Maren, Pickles, Isabelle B., Sobott, Frank, Sivaprasadarao, Asipu, Postis, Vincent L.G., Muench, Stephen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086155/
https://www.ncbi.nlm.nih.gov/pubmed/31945320
http://dx.doi.org/10.1016/j.bbamem.2020.183192