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Styrene maleic-acid lipid particles (SMALPs) into detergent or amphipols: An exchange protocol for membrane protein characterisation
Membrane proteins are traditionally extracted and purified in detergent for biochemical and structural characterisation. This process is often costly and laborious, and the stripping away of potentially stabilising lipids from the membrane protein of interest can have detrimental effects on protein...
Autores principales: | Hesketh, Sophie J., Klebl, David P., Higgins, Anna J., Thomsen, Maren, Pickles, Isabelle B., Sobott, Frank, Sivaprasadarao, Asipu, Postis, Vincent L.G., Muench, Stephen P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086155/ https://www.ncbi.nlm.nih.gov/pubmed/31945320 http://dx.doi.org/10.1016/j.bbamem.2020.183192 |
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