A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis

This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow fiber-membrane module and...

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Detalles Bibliográficos
Autores principales: Zhu, Kaichun, Jin, Huali, He, Zhonghuai, Zhu, Qinghong, Wang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086510/
https://www.ncbi.nlm.nih.gov/pubmed/17406572
http://dx.doi.org/10.1038/nprot.2006.452

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086510/
https://www.ncbi.nlm.nih.gov/pubmed/17406572
http://dx.doi.org/10.1038/nprot.2006.452

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