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Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5′-untranslated sequence wer...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086588/ https://www.ncbi.nlm.nih.gov/pubmed/12111435 http://dx.doi.org/10.1007/s00705-002-0808-4 |
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author | Matsumura, T. Itchoda, N. Tsunemitsu, H. |
author_facet | Matsumura, T. Itchoda, N. Tsunemitsu, H. |
author_sort | Matsumura, T. |
collection | PubMed |
description | We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5′-untranslated sequence were positive for GAR antigens by ELISA. The expressed protein was consistent in size with VP6 of GAR by Western blot assay. The presence of the VP6 gene and its transcript was detected by PCR and RT-PCR. Adult BALB/c mice were immunized intraperitoneally with concentrated transgenic potato extracts emulsified in Freund’s adjuvant. Sera collected after immunization showed the anti-VP6 response in ELISA and Western blot assay. These results suggest that the immunogenic VP6 protein expressed in plants could be useful for the preparation of diagnostic reagents. |
format | Online Article Text |
id | pubmed-7086588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-70865882020-03-23 Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants Matsumura, T. Itchoda, N. Tsunemitsu, H. Arch Virol Brief Report We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5′-untranslated sequence were positive for GAR antigens by ELISA. The expressed protein was consistent in size with VP6 of GAR by Western blot assay. The presence of the VP6 gene and its transcript was detected by PCR and RT-PCR. Adult BALB/c mice were immunized intraperitoneally with concentrated transgenic potato extracts emulsified in Freund’s adjuvant. Sera collected after immunization showed the anti-VP6 response in ELISA and Western blot assay. These results suggest that the immunogenic VP6 protein expressed in plants could be useful for the preparation of diagnostic reagents. Springer-Verlag 2014-02-08 2002 /pmc/articles/PMC7086588/ /pubmed/12111435 http://dx.doi.org/10.1007/s00705-002-0808-4 Text en © Springer-Verlag/Wien 2002 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Brief Report Matsumura, T. Itchoda, N. Tsunemitsu, H. Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants |
title | Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants |
title_full | Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants |
title_fullStr | Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants |
title_full_unstemmed | Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants |
title_short | Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants |
title_sort | production of immunogenic vp6 protein of bovine group a rotavirus in transgenic potato plants |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086588/ https://www.ncbi.nlm.nih.gov/pubmed/12111435 http://dx.doi.org/10.1007/s00705-002-0808-4 |
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