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Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants

 We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5′-untranslated sequence wer...

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Autores principales: Matsumura, T., Itchoda, N., Tsunemitsu, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086588/
https://www.ncbi.nlm.nih.gov/pubmed/12111435
http://dx.doi.org/10.1007/s00705-002-0808-4
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author Matsumura, T.
Itchoda, N.
Tsunemitsu, H.
author_facet Matsumura, T.
Itchoda, N.
Tsunemitsu, H.
author_sort Matsumura, T.
collection PubMed
description  We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5′-untranslated sequence were positive for GAR antigens by ELISA. The expressed protein was consistent in size with VP6 of GAR by Western blot assay. The presence of the VP6 gene and its transcript was detected by PCR and RT-PCR. Adult BALB/c mice were immunized intraperitoneally with concentrated transgenic potato extracts emulsified in Freund’s adjuvant. Sera collected after immunization showed the anti-VP6 response in ELISA and Western blot assay. These results suggest that the immunogenic VP6 protein expressed in plants could be useful for the preparation of diagnostic reagents.
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spelling pubmed-70865882020-03-23 Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants Matsumura, T. Itchoda, N. Tsunemitsu, H. Arch Virol Brief Report  We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5′-untranslated sequence were positive for GAR antigens by ELISA. The expressed protein was consistent in size with VP6 of GAR by Western blot assay. The presence of the VP6 gene and its transcript was detected by PCR and RT-PCR. Adult BALB/c mice were immunized intraperitoneally with concentrated transgenic potato extracts emulsified in Freund’s adjuvant. Sera collected after immunization showed the anti-VP6 response in ELISA and Western blot assay. These results suggest that the immunogenic VP6 protein expressed in plants could be useful for the preparation of diagnostic reagents. Springer-Verlag 2014-02-08 2002 /pmc/articles/PMC7086588/ /pubmed/12111435 http://dx.doi.org/10.1007/s00705-002-0808-4 Text en © Springer-Verlag/Wien 2002 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Matsumura, T.
Itchoda, N.
Tsunemitsu, H.
Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
title Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
title_full Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
title_fullStr Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
title_full_unstemmed Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
title_short Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants
title_sort production of immunogenic vp6 protein of bovine group a rotavirus in transgenic potato plants
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086588/
https://www.ncbi.nlm.nih.gov/pubmed/12111435
http://dx.doi.org/10.1007/s00705-002-0808-4
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