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Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR
A reverse transcriptase PCR (RT-PCR) targeting a 407 bp fragment of the nucleocapsid gene of bovine coronavirus (BCV) was developed for detection of BCV RNA in feces of experimentally inoculated cattle. The sensitivity and specificity of the RT-PCR were confirmed using tissue culture-adapted BCV str...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer-Verlag
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086631/ https://www.ncbi.nlm.nih.gov/pubmed/10076517 http://dx.doi.org/10.1007/s007050050493 |