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Development of a highly sensitive real-time nested RT-PCR assay in a single closed tube for detection of enterovirus 71 in hand, foot, and mouth disease

Enterovirus 71 (EV71) is one of the major causative agents of outbreaks of hand, foot, and mouth disease (HFMD). A commercial TaqMan probe-based real-time PCR assay has been widely used for the differential detection of EV71 despite its relatively high cost and failure to detect samples with a low v...

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Detalles Bibliográficos
Autores principales: Niu, Peihua, Qi, Shunxiang, Yu, Benzhang, Zhang, Chen, Wang, Ji, Li, Qi, Ma, Xuejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086773/
https://www.ncbi.nlm.nih.gov/pubmed/27475103
http://dx.doi.org/10.1007/s00705-016-2985-6