Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein

The complete nonstructural NS5 gene of Japanese encephalitis virus (JEV) was amplified and cloned into an expression vector. The NS5 protein was expressed in Escherichia coli and purified by His-tag based affinity chromatography. This recombinant NS5 protein exhibited RNA-dependent RNA polymerase (R...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, F., Hasebe, F., Inoue, S., Mathenge, E. G. M., Morita, K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086876/
https://www.ncbi.nlm.nih.gov/pubmed/17577613
http://dx.doi.org/10.1007/s00705-007-1007-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086876/
https://www.ncbi.nlm.nih.gov/pubmed/17577613
http://dx.doi.org/10.1007/s00705-007-1007-0

Ejemplares similares