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A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism

Two primers with the length of 22 bases each and 400 bases apart on the spike protein gene of avian infectious bronchitis virus (IBV) were prepared. Using these primers, the genome RNA from twelve strains of the various serotypes were reverse-transcribed to cDNA and amplified by polymerase chain rea...

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Detalles Bibliográficos
Autores principales: Lin, Z., Kato, A., Kudou, Y., Ueda, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1991
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086981/
https://www.ncbi.nlm.nih.gov/pubmed/1672064
http://dx.doi.org/10.1007/BF01319228