Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI

Infectious bronchitis virus (IBV) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. A virulent strain of IBV, isolated from Arkansas (Ark), was propagated in embryonated eggs (Ark DPI 11)...

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Autores principales: Ammayappan, Arun, Upadhyay, Chitra, Gelb, Jack, Vakharia, Vikram N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086983/
https://www.ncbi.nlm.nih.gov/pubmed/19219402
http://dx.doi.org/10.1007/s00705-009-0325-9
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author Ammayappan, Arun
Upadhyay, Chitra
Gelb, Jack
Vakharia, Vikram N.
author_facet Ammayappan, Arun
Upadhyay, Chitra
Gelb, Jack
Vakharia, Vikram N.
author_sort Ammayappan, Arun
collection PubMed
description Infectious bronchitis virus (IBV) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. A virulent strain of IBV, isolated from Arkansas (Ark), was propagated in embryonated eggs (Ark DPI 11). Following 101 serial passages in embryonated eggs, an attenuated strain of IBV was established (Ark DPI 101) that does not induce histopathological lesions in the tracheae of infected chicks. To identify sequence changes responsible for the attenuation of IBV, complete genome sequences of both virulent and attenuated Ark DPI viruses were obtained. Comparison of the genome sequences of the virulent and attenuated Ark DPI viruses reveals that these viruses are similar and differ only by 21 nucleotides, resulting in 17 amino acids changes. Most of those substitutions are located in the replicase 1a and spike genes. No differences were observed in gene 3, M or 5a, and only one nucleotide substitution each was present in 5b, N and 3′UTR. By comparing the deduced amino acid sequences of virulent and attenuated viruses, we identified sequence changes responsible for the adaptation and attenuation of the IBV-Ark DPI strain.
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spelling pubmed-70869832020-03-23 Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI Ammayappan, Arun Upadhyay, Chitra Gelb, Jack Vakharia, Vikram N. Arch Virol Brief Report Infectious bronchitis virus (IBV) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. A virulent strain of IBV, isolated from Arkansas (Ark), was propagated in embryonated eggs (Ark DPI 11). Following 101 serial passages in embryonated eggs, an attenuated strain of IBV was established (Ark DPI 101) that does not induce histopathological lesions in the tracheae of infected chicks. To identify sequence changes responsible for the attenuation of IBV, complete genome sequences of both virulent and attenuated Ark DPI viruses were obtained. Comparison of the genome sequences of the virulent and attenuated Ark DPI viruses reveals that these viruses are similar and differ only by 21 nucleotides, resulting in 17 amino acids changes. Most of those substitutions are located in the replicase 1a and spike genes. No differences were observed in gene 3, M or 5a, and only one nucleotide substitution each was present in 5b, N and 3′UTR. By comparing the deduced amino acid sequences of virulent and attenuated viruses, we identified sequence changes responsible for the adaptation and attenuation of the IBV-Ark DPI strain. Springer Vienna 2009-02-14 2009 /pmc/articles/PMC7086983/ /pubmed/19219402 http://dx.doi.org/10.1007/s00705-009-0325-9 Text en © Springer-Verlag 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Ammayappan, Arun
Upadhyay, Chitra
Gelb, Jack
Vakharia, Vikram N.
Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
title Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
title_full Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
title_fullStr Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
title_full_unstemmed Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
title_short Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI
title_sort identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain arkansas dpi
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086983/
https://www.ncbi.nlm.nih.gov/pubmed/19219402
http://dx.doi.org/10.1007/s00705-009-0325-9
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