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Cross-protection studies between respiratory and calf diarrhea and winter dysentery coronavirus strains in calves and RT-PCR and nested PCR for their detection

 A 1-step RT-PCR assay, targeting a 730 bp fragment of the nucleocapsid (N) gene of bovine coronavirus (BCV), and a nested PCR assay, targeting a 407 bp fragment of the N gene, were developed to detect BCV in nasal swab and fecal samples of calves experimentally exposed to BCV. Both 1-step RT-PCR an...

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Detalles Bibliográficos
Autores principales: Cho, K.-O., Hasoksuz, M., Nielsen, P. R., Chang, K.-O., Lathrop, S., Saif, L. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087283/
https://www.ncbi.nlm.nih.gov/pubmed/11811688
http://dx.doi.org/10.1007/s007050170011