Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductases from higher plants

Messenger RNAs encoding the nitrate reductase apoenzyme from tobacco can be translated in a cell-free system. Poly(A)(+) mRNA fractions from the 23-32 S area of a sucrose gradient were used to build a cDNA library in the expression vector λgt11 with an efficiency of cloning of approximately 10(4) re...

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Detalles Bibliográficos
Autores principales: Calza, Roger, Huttner, Eric, Vincentz, Michel, Rouzé, Pierre, Galangau, Fabienne, Vaucheret, Hervé, Chérel, Isabelle, Meyer, Christian, Kronenberger, Jocelyne, Caboche, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1987
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087718/
https://www.ncbi.nlm.nih.gov/pubmed/17193712
http://dx.doi.org/10.1007/BF00331162

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087718/
https://www.ncbi.nlm.nih.gov/pubmed/17193712
http://dx.doi.org/10.1007/BF00331162

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