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Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death k...

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Detalles Bibliográficos
Autores principales: Lai, Chia-Wei, Chung, Yao-Chi, Lai, Yiu-Kay, Chang, Margaret Dah-Tsyr, Hu, Yu-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087856/
https://www.ncbi.nlm.nih.gov/pubmed/16091881
http://dx.doi.org/10.1007/s10529-005-7176-6