Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death k...

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Detalles Bibliográficos
Autores principales: Lai, Chia-Wei, Chung, Yao-Chi, Lai, Yiu-Kay, Chang, Margaret Dah-Tsyr, Hu, Yu-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087856/
https://www.ncbi.nlm.nih.gov/pubmed/16091881
http://dx.doi.org/10.1007/s10529-005-7176-6
Descripción
Sumario:Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.

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