Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death k...

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Autores principales: Lai, Chia-Wei, Chung, Yao-Chi, Lai, Yiu-Kay, Chang, Margaret Dah-Tsyr, Hu, Yu-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087856/
https://www.ncbi.nlm.nih.gov/pubmed/16091881
http://dx.doi.org/10.1007/s10529-005-7176-6
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author Lai, Chia-Wei
Chung, Yao-Chi
Lai, Yiu-Kay
Chang, Margaret Dah-Tsyr
Hu, Yu-Chen
author_facet Lai, Chia-Wei
Chung, Yao-Chi
Lai, Yiu-Kay
Chang, Margaret Dah-Tsyr
Hu, Yu-Chen
author_sort Lai, Chia-Wei
collection PubMed
description Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.
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spelling pubmed-70878562020-03-23 Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study Lai, Chia-Wei Chung, Yao-Chi Lai, Yiu-Kay Chang, Margaret Dah-Tsyr Hu, Yu-Chen Biotechnol Lett Article Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed. Kluwer Academic Publishers 2005 /pmc/articles/PMC7087856/ /pubmed/16091881 http://dx.doi.org/10.1007/s10529-005-7176-6 Text en © Springer 2005 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Lai, Chia-Wei
Chung, Yao-Chi
Lai, Yiu-Kay
Chang, Margaret Dah-Tsyr
Hu, Yu-Chen
Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study
title Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study
title_full Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study
title_fullStr Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study
title_full_unstemmed Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study
title_short Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study
title_sort expression and purification of n and e proteins from severe acute respiratory syndrome (sars)-associated coronavirus: a comparative study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087856/
https://www.ncbi.nlm.nih.gov/pubmed/16091881
http://dx.doi.org/10.1007/s10529-005-7176-6
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