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One-Tube Nested RT-PCR Enabled by Using a Plastic Film and its Application for the Rapid Detection of SARS-Virus
A general strategy based on the use of a plastic film that allows reverse transcription and nested-PCR in a single closed-tube has been developed. The reaction mixture for the second PCR amplification is quarantined in the cap of the reaction tube during the first round amplification by a piece of p...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Kluwer Academic Publishers
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087936/ https://www.ncbi.nlm.nih.gov/pubmed/15049359 http://dx.doi.org/10.1023/B:BILE.0000013708.65032.f1 |