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Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection

A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the...

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Detalles Bibliográficos
Autores principales: Ren, Xiaofeng, Suo, Siqingaowa, Jang, Yong-Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088053/
https://www.ncbi.nlm.nih.gov/pubmed/20882317
http://dx.doi.org/10.1007/s10529-010-0420-8