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Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection
A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088053/ https://www.ncbi.nlm.nih.gov/pubmed/20882317 http://dx.doi.org/10.1007/s10529-010-0420-8 |