Immobilization of Ulp1 protease on NHS-activated Sepharose: a useful tool for cleavage of the SUMO tag of recombinant proteins

OBJECTIVE: To fabricate an active and stable enzyme through covalent immobilization, a Ubl-specific protease (Ulp1) was used to cleave small ubiquitin-like modifier (SUMO) fusion proteins. RESULTS: We immobilized Ulp1 on N-hydroxysuccinimide (NHS)-activated Sepharose with a coupling efficiency of 1....

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Detalles Bibliográficos
Autores principales: Liang, Qiujin, Huang, Zhengzhi, Zhang, Yuan, Li, Hongtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088063/
https://www.ncbi.nlm.nih.gov/pubmed/28432497
http://dx.doi.org/10.1007/s10529-017-2330-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088063/
https://www.ncbi.nlm.nih.gov/pubmed/28432497
http://dx.doi.org/10.1007/s10529-017-2330-5

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