Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates

A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.

Detalles Bibliográficos
Autores principales: Polster, Brian M., Arze, Rafael, Lyttle, Matthew H., Nicholls, David G., Hudson, Derek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 2006
Materias:
Bruce Merrifield Commemorative Issue
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088158/
https://www.ncbi.nlm.nih.gov/pubmed/32214925
http://dx.doi.org/10.1007/s10989-006-9049-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088158/
https://www.ncbi.nlm.nih.gov/pubmed/32214925
http://dx.doi.org/10.1007/s10989-006-9049-9

Ejemplares similares