Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates

A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.

Detalles Bibliográficos
Autores principales: Polster, Brian M., Arze, Rafael, Lyttle, Matthew H., Nicholls, David G., Hudson, Derek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 2006
Materias:
Bruce Merrifield Commemorative Issue
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088158/
https://www.ncbi.nlm.nih.gov/pubmed/32214925
http://dx.doi.org/10.1007/s10989-006-9049-9
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author Polster, Brian M.
Arze, Rafael
Lyttle, Matthew H.
Nicholls, David G.
Hudson, Derek
author_facet Polster, Brian M.
Arze, Rafael
Lyttle, Matthew H.
Nicholls, David G.
Hudson, Derek
author_sort Polster, Brian M.
collection PubMed
description A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.
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spelling pubmed-70881582020-03-23 Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates Polster, Brian M. Arze, Rafael Lyttle, Matthew H. Nicholls, David G. Hudson, Derek Int J Pept Res Ther Bruce Merrifield Commemorative Issue A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes. Kluwer Academic Publishers 2006-11-30 2007 /pmc/articles/PMC7088158/ /pubmed/32214925 http://dx.doi.org/10.1007/s10989-006-9049-9 Text en © Springer Science+Business Media, Inc. 2006 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Bruce Merrifield Commemorative Issue
Polster, Brian M.
Arze, Rafael
Lyttle, Matthew H.
Nicholls, David G.
Hudson, Derek
Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
title Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
title_full Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
title_fullStr Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
title_full_unstemmed Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
title_short Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
title_sort solid phase synthesis of dual labeled peptides: development of cell permeable calpain specific substrates
topic Bruce Merrifield Commemorative Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088158/
https://www.ncbi.nlm.nih.gov/pubmed/32214925
http://dx.doi.org/10.1007/s10989-006-9049-9
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