Using DNA Shuffling to Create Novel Infectious Bronchitis Virus S1 Genes: Implications for S1 Gene Recombination

We employed the staggered extension process (StEP) to shuffle the S1 genes from four infectious bronchitis virus (IBV) strains representing four unique serotypes. Upon creating a shuffled S1 gene library, we randomly selected 25 clones and analyzed them by DNA sequencing. In total, eleven clones con...

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Detalles Bibliográficos
Autores principales: Callison, Scott, Hilt, Deborah, Jackwood, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088642/
https://www.ncbi.nlm.nih.gov/pubmed/15965603
http://dx.doi.org/10.1007/s11262-004-2194-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088642/
https://www.ncbi.nlm.nih.gov/pubmed/15965603
http://dx.doi.org/10.1007/s11262-004-2194-3

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