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Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions
Amplifying the variable (Fv or V) regions of immunoglobulins (Ig) has become a challenge in cloning antibody genes for phage display, a technique used to study protein–protein, protein–peptide, and protein–DNA interactions using bacteriophages to connect proteins with the genetic information that en...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089251/ https://www.ncbi.nlm.nih.gov/pubmed/22806814 http://dx.doi.org/10.1007/s11274-011-0830-3 |