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Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions

Amplifying the variable (Fv or V) regions of immunoglobulins (Ig) has become a challenge in cloning antibody genes for phage display, a technique used to study protein–protein, protein–peptide, and protein–DNA interactions using bacteriophages to connect proteins with the genetic information that en...

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Detalles Bibliográficos
Autores principales:	Sun, Ying, Liu, Hong-Yan, Mu, Ling, Luo, En-Jie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2011
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089251/ https://www.ncbi.nlm.nih.gov/pubmed/22806814 http://dx.doi.org/10.1007/s11274-011-0830-3

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