Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometry

The technical difficulty to isolate microglia, astrocytes and infiltrating immune cells from mouse brain is nowadays a limiting factor in the study of neuroinflammation. Brain isolation requirements are cell-type and animal-age dependent, but current brain dissociation procedures are poorly standard...

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Detalles Bibliográficos
Autores principales: Calvo, Belén, Rubio, Felipe, Fernández, Miriam, Tranque, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090101/
https://www.ncbi.nlm.nih.gov/pubmed/32215337
http://dx.doi.org/10.1016/j.ibror.2019.12.004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090101/
https://www.ncbi.nlm.nih.gov/pubmed/32215337
http://dx.doi.org/10.1016/j.ibror.2019.12.004

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