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Precise large deletions by the PCR-based overlap extension method
The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparat...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Humana Press
1995
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090436/ https://www.ncbi.nlm.nih.gov/pubmed/8521036 http://dx.doi.org/10.1007/BF02907467 |