Precise large deletions by the PCR-based overlap extension method

The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparat...

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Detalles Bibliográficos
Autores principales: Senanayake, Savithra D., Brian, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Humana Press 1995
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090436/
https://www.ncbi.nlm.nih.gov/pubmed/8521036
http://dx.doi.org/10.1007/BF02907467
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author Senanayake, Savithra D.
Brian, David A.
author_facet Senanayake, Savithra D.
Brian, David A.
author_sort Senanayake, Savithra D.
collection PubMed
description The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
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spelling pubmed-70904362020-03-24 Precise large deletions by the PCR-based overlap extension method Senanayake, Savithra D. Brian, David A. Mol Biotechnol Protocol The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template. Humana Press 1995 /pmc/articles/PMC7090436/ /pubmed/8521036 http://dx.doi.org/10.1007/BF02907467 Text en © Humana Press Inc 1995 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Protocol
Senanayake, Savithra D.
Brian, David A.
Precise large deletions by the PCR-based overlap extension method
title Precise large deletions by the PCR-based overlap extension method
title_full Precise large deletions by the PCR-based overlap extension method
title_fullStr Precise large deletions by the PCR-based overlap extension method
title_full_unstemmed Precise large deletions by the PCR-based overlap extension method
title_short Precise large deletions by the PCR-based overlap extension method
title_sort precise large deletions by the pcr-based overlap extension method
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090436/
https://www.ncbi.nlm.nih.gov/pubmed/8521036
http://dx.doi.org/10.1007/BF02907467
work_keys_str_mv AT senanayakesavithrad preciselargedeletionsbythepcrbasedoverlapextensionmethod
AT briandavida preciselargedeletionsbythepcrbasedoverlapextensionmethod

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