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Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid
The antiviral potential of selected bacteria species [lactic acid bacteria (LAB) and micrococcaceae] was examined. By this, the effect of their cell-free supernatants as well as of certain species-related metabolites (sakacin A, nisin, and lactic acid) was investigated on different viruses after exp...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090810/ https://www.ncbi.nlm.nih.gov/pubmed/24297091 http://dx.doi.org/10.1007/s12560-013-9135-z |
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author | Lange-Starke, Anett Petereit, A. Truyen, U. Braun, P. G. Fehlhaber, K. Albert, T. |
author_facet | Lange-Starke, Anett Petereit, A. Truyen, U. Braun, P. G. Fehlhaber, K. Albert, T. |
author_sort | Lange-Starke, Anett |
collection | PubMed |
description | The antiviral potential of selected bacteria species [lactic acid bacteria (LAB) and micrococcaceae] was examined. By this, the effect of their cell-free supernatants as well as of certain species-related metabolites (sakacin A, nisin, and lactic acid) was investigated on different viruses after exposure at 24 °C for 3 days. Viruses were incubated with supernatants and metabolites in a dilution ratio of 1:10. Data for antiviral effects towards murine norovirus S99 (MNV), influenza A virus A/WSN/33 (H1N1), Newcastle disease virus Montana (NDV) and feline herpesvirus KS 285 (FHV) were generated in vitro simulating pH and temperature conditions according to raw sausage fermentations. Investigations showed no antiviral effect of sakacin A and nisin on MNV, H1N1, FHV and NDV. Furthermore, the antiviral potential of d,l-lactic acid was determined for MNV and H1N1. At raw sausage-related pH values (5.0–6.2) it could be shown that the virus titre for MNV and H1N1 was reduced by a maximum of 3.25 log and 2.5 log units, respectively. In addition, 29 culture supernatants of different bacteria species, mainly LAB and staphylococci, were tested for their antiviral activity against MNV. Only the cell-free supernatant of a Lb. curvatus strain showed a higher virus titre reduction of MNV by 1.25 log units compared to the control. Further studies on the characterisation of this cell-free supernatant were carried out, however, the antiviral substance could not be identified so far. |
format | Online Article Text |
id | pubmed-7090810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-70908102020-03-24 Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid Lange-Starke, Anett Petereit, A. Truyen, U. Braun, P. G. Fehlhaber, K. Albert, T. Food Environ Virol Original Paper The antiviral potential of selected bacteria species [lactic acid bacteria (LAB) and micrococcaceae] was examined. By this, the effect of their cell-free supernatants as well as of certain species-related metabolites (sakacin A, nisin, and lactic acid) was investigated on different viruses after exposure at 24 °C for 3 days. Viruses were incubated with supernatants and metabolites in a dilution ratio of 1:10. Data for antiviral effects towards murine norovirus S99 (MNV), influenza A virus A/WSN/33 (H1N1), Newcastle disease virus Montana (NDV) and feline herpesvirus KS 285 (FHV) were generated in vitro simulating pH and temperature conditions according to raw sausage fermentations. Investigations showed no antiviral effect of sakacin A and nisin on MNV, H1N1, FHV and NDV. Furthermore, the antiviral potential of d,l-lactic acid was determined for MNV and H1N1. At raw sausage-related pH values (5.0–6.2) it could be shown that the virus titre for MNV and H1N1 was reduced by a maximum of 3.25 log and 2.5 log units, respectively. In addition, 29 culture supernatants of different bacteria species, mainly LAB and staphylococci, were tested for their antiviral activity against MNV. Only the cell-free supernatant of a Lb. curvatus strain showed a higher virus titre reduction of MNV by 1.25 log units compared to the control. Further studies on the characterisation of this cell-free supernatant were carried out, however, the antiviral substance could not be identified so far. Springer US 2013-12-03 2014 /pmc/articles/PMC7090810/ /pubmed/24297091 http://dx.doi.org/10.1007/s12560-013-9135-z Text en © Springer Science+Business Media New York 2013 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Lange-Starke, Anett Petereit, A. Truyen, U. Braun, P. G. Fehlhaber, K. Albert, T. Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid |
title | Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid |
title_full | Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid |
title_fullStr | Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid |
title_full_unstemmed | Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid |
title_short | Antiviral Potential of Selected Starter Cultures, Bacteriocins and d,l-Lactic Acid |
title_sort | antiviral potential of selected starter cultures, bacteriocins and d,l-lactic acid |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090810/ https://www.ncbi.nlm.nih.gov/pubmed/24297091 http://dx.doi.org/10.1007/s12560-013-9135-z |
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