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Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
AIM: To characterize enzymatic activity of severe acute respiratory syndrome (SARS) coronavirus (CoV) 3C-like protease (3CL(pro)) and its four site-directed mutants. METHODS: Based on the fluorescence resonance energy transfer (FRET) principle using 5-[(2′-aminoethyl)-amino] naphthelenesulfonic acid...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091904/ https://www.ncbi.nlm.nih.gov/pubmed/15659121 http://dx.doi.org/10.1111/j.1745-7254.2005.00010.x |