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Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis

BACKGROUND: Oligomannuronates (OM) are natural products from alginate that is frequently used as food supplement. The aim of this study was to investigate the in vitro protective effects of OM on RINm5F cells against human Islet amyloid polypeptide (IAPP) induced mitochondrial dysfunction, as well a...

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Autores principales: Liu, Xi, Li, Qiong, Cheng, Xiaolei, Liu, Zhichun, Zhao, Xiaoliang, Zhang, Shuai, Yu, Guangli, Zhao, Xia, Hao, Jiejie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092590/
https://www.ncbi.nlm.nih.gov/pubmed/32226477
http://dx.doi.org/10.1186/s13020-020-00310-4
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author Liu, Xi
Li, Qiong
Cheng, Xiaolei
Liu, Zhichun
Zhao, Xiaoliang
Zhang, Shuai
Yu, Guangli
Zhao, Xia
Hao, Jiejie
author_facet Liu, Xi
Li, Qiong
Cheng, Xiaolei
Liu, Zhichun
Zhao, Xiaoliang
Zhang, Shuai
Yu, Guangli
Zhao, Xia
Hao, Jiejie
author_sort Liu, Xi
collection PubMed
description BACKGROUND: Oligomannuronates (OM) are natural products from alginate that is frequently used as food supplement. The aim of this study was to investigate the in vitro protective effects of OM on RINm5F cells against human Islet amyloid polypeptide (IAPP) induced mitochondrial dysfunction, as well as the underlying mechanisms. METHODS: In the present study, we obtained several kinds of OM with different molecular masses, and then we used RINm5F cells as a model to elucidate the involvement of JNK signal pathway in hIAPP-induced mitochondrial dysfunction in pancreatic beta cells, and the protective effects of OM are associated with its ability to attenuate the mitochondrial dysfunction. RESULTS: Our results demonstrated that human IAPP induced mitochondrial dysfunction, as evidence by loss of ΔΨm and ATP content, and decrease in oxygen consumption and complex activities, was accompanied by JNK activation, changes in the expressions of Bcl-2 and Bax proteins, release of cytochrome c (Cyto-c) and apoptosis inducing factor (AIF) from mitochondria into cytosol. Interestingly, the human IAPP induced damage in RINm5F cells were effectively restored by co-treatment of OM. Moreover, JNK activation was required for the OM mediated changes in RINm5F cells. CONCLUSIONS: OM prevented mitochondrial dysfunction induced by human IAPP in RINm5F islet cells through JNK dependent signaling pathways.
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spelling pubmed-70925902020-03-27 Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis Liu, Xi Li, Qiong Cheng, Xiaolei Liu, Zhichun Zhao, Xiaoliang Zhang, Shuai Yu, Guangli Zhao, Xia Hao, Jiejie Chin Med Research BACKGROUND: Oligomannuronates (OM) are natural products from alginate that is frequently used as food supplement. The aim of this study was to investigate the in vitro protective effects of OM on RINm5F cells against human Islet amyloid polypeptide (IAPP) induced mitochondrial dysfunction, as well as the underlying mechanisms. METHODS: In the present study, we obtained several kinds of OM with different molecular masses, and then we used RINm5F cells as a model to elucidate the involvement of JNK signal pathway in hIAPP-induced mitochondrial dysfunction in pancreatic beta cells, and the protective effects of OM are associated with its ability to attenuate the mitochondrial dysfunction. RESULTS: Our results demonstrated that human IAPP induced mitochondrial dysfunction, as evidence by loss of ΔΨm and ATP content, and decrease in oxygen consumption and complex activities, was accompanied by JNK activation, changes in the expressions of Bcl-2 and Bax proteins, release of cytochrome c (Cyto-c) and apoptosis inducing factor (AIF) from mitochondria into cytosol. Interestingly, the human IAPP induced damage in RINm5F cells were effectively restored by co-treatment of OM. Moreover, JNK activation was required for the OM mediated changes in RINm5F cells. CONCLUSIONS: OM prevented mitochondrial dysfunction induced by human IAPP in RINm5F islet cells through JNK dependent signaling pathways. BioMed Central 2020-03-24 /pmc/articles/PMC7092590/ /pubmed/32226477 http://dx.doi.org/10.1186/s13020-020-00310-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Xi
Li, Qiong
Cheng, Xiaolei
Liu, Zhichun
Zhao, Xiaoliang
Zhang, Shuai
Yu, Guangli
Zhao, Xia
Hao, Jiejie
Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
title Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
title_full Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
title_fullStr Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
title_full_unstemmed Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
title_short Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
title_sort oligomannuronate prevents mitochondrial dysfunction induced by iapp in rinm5f islet cells by inhibition of jnk activation and cell apoptosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092590/
https://www.ncbi.nlm.nih.gov/pubmed/32226477
http://dx.doi.org/10.1186/s13020-020-00310-4
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