Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input

We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and...

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Detalles Bibliográficos
Autores principales: Daigneault, Bradford W, Rajput, Sandeep K, Smith, George W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Benchmark
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092705/
https://www.ncbi.nlm.nih.gov/pubmed/31937114
http://dx.doi.org/10.2144/btn-2019-0121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092705/
https://www.ncbi.nlm.nih.gov/pubmed/31937114
http://dx.doi.org/10.2144/btn-2019-0121

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