Cargando…
Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input
We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092705/ https://www.ncbi.nlm.nih.gov/pubmed/31937114 http://dx.doi.org/10.2144/btn-2019-0121 |
_version_ | 1783510156999393280 |
---|---|
author | Daigneault, Bradford W Rajput, Sandeep K Smith, George W |
author_facet | Daigneault, Bradford W Rajput, Sandeep K Smith, George W |
author_sort | Daigneault, Bradford W |
collection | PubMed |
description | We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and agriculture, including clinical diagnoses of infertility, the identification of single-nucleotide polymorphisms and aberrant methylation patterns that can impact fertility, lower embryo development and contribute to heritable disease. The methods described here provide a reliable, simplistic approach for analyzing both the genomic and epigenomic status of whole sperm ejaculates that can be adapted for laboratory diagnostics, clinical reproductive practice and basic research. |
format | Online Article Text |
id | pubmed-7092705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-70927052020-03-24 Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input Daigneault, Bradford W Rajput, Sandeep K Smith, George W Biotechniques Benchmark We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and agriculture, including clinical diagnoses of infertility, the identification of single-nucleotide polymorphisms and aberrant methylation patterns that can impact fertility, lower embryo development and contribute to heritable disease. The methods described here provide a reliable, simplistic approach for analyzing both the genomic and epigenomic status of whole sperm ejaculates that can be adapted for laboratory diagnostics, clinical reproductive practice and basic research. Future Science Ltd 2020-01-16 2019-12 /pmc/articles/PMC7092705/ /pubmed/31937114 http://dx.doi.org/10.2144/btn-2019-0121 Text en © 2020 Bradford W. Daigneault This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Benchmark Daigneault, Bradford W Rajput, Sandeep K Smith, George W Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
title | Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
title_full | Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
title_fullStr | Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
title_full_unstemmed | Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
title_short | Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
title_sort | simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input |
topic | Benchmark |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092705/ https://www.ncbi.nlm.nih.gov/pubmed/31937114 http://dx.doi.org/10.2144/btn-2019-0121 |
work_keys_str_mv | AT daigneaultbradfordw simpleworkflowforgenomeandmethylationanalysesofejaculatedbovinespermatozoawithlowsperminput AT rajputsandeepk simpleworkflowforgenomeandmethylationanalysesofejaculatedbovinespermatozoawithlowsperminput AT smithgeorgew simpleworkflowforgenomeandmethylationanalysesofejaculatedbovinespermatozoawithlowsperminput |