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Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input
We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Future Science Ltd
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092705/ https://www.ncbi.nlm.nih.gov/pubmed/31937114 http://dx.doi.org/10.2144/btn-2019-0121 |