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An improved shotgun antisense method for mutagenesis and gene identification

Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in Dictyostelium discoideum. However, the method has two limitations. First, there were too few clones in the shotgun antisense cDNA library t...

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Detalles Bibliográficos
Autores principales: Tang, Yu, Gomer, Richard H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092706/
https://www.ncbi.nlm.nih.gov/pubmed/31973564
http://dx.doi.org/10.2144/btn-2019-0123