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Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier
Despite the controversy regarding the existence and physiological relevance of class A G protein-coupled receptor dimerization, there is substantial evidence for functional interactions between the dopamine D2 receptor (D2R) and the adenosine A2A receptor (A2AR). A2AR-D2R complexes have been detecte...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092707/ https://www.ncbi.nlm.nih.gov/pubmed/31859535 http://dx.doi.org/10.2144/btn-2019-0083 |
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author | Zhu, Ying Mészáros, József Walle, Roman Fan, Rongxi Sun, Ziyi Dwork, Andrew J Trifilieff, Pierre Javitch, Jonathan A |
author_facet | Zhu, Ying Mészáros, József Walle, Roman Fan, Rongxi Sun, Ziyi Dwork, Andrew J Trifilieff, Pierre Javitch, Jonathan A |
author_sort | Zhu, Ying |
collection | PubMed |
description | Despite the controversy regarding the existence and physiological relevance of class A G protein-coupled receptor dimerization, there is substantial evidence for functional interactions between the dopamine D2 receptor (D2R) and the adenosine A2A receptor (A2AR). A2AR-D2R complexes have been detected in rodent brains by proximity ligation assay; however, their existence in the human brain has not been demonstrated. In this study, we used Brightfield proximity ligation assay, combined with a systematic sampling and a parameter-free naive Bayesian classifier, and demonstrated proximity between the D2R and the A2AR in the adult human ventral striatum, consistent with their colocalization within complexes and the possible existence of D2R-A2AR heteromers. These methods are applicable to the relative quantification of proximity of two proteins, as well as the expression levels of individual proteins. |
format | Online Article Text |
id | pubmed-7092707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-70927072020-03-24 Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier Zhu, Ying Mészáros, József Walle, Roman Fan, Rongxi Sun, Ziyi Dwork, Andrew J Trifilieff, Pierre Javitch, Jonathan A Biotechniques Reports Despite the controversy regarding the existence and physiological relevance of class A G protein-coupled receptor dimerization, there is substantial evidence for functional interactions between the dopamine D2 receptor (D2R) and the adenosine A2A receptor (A2AR). A2AR-D2R complexes have been detected in rodent brains by proximity ligation assay; however, their existence in the human brain has not been demonstrated. In this study, we used Brightfield proximity ligation assay, combined with a systematic sampling and a parameter-free naive Bayesian classifier, and demonstrated proximity between the D2R and the A2AR in the adult human ventral striatum, consistent with their colocalization within complexes and the possible existence of D2R-A2AR heteromers. These methods are applicable to the relative quantification of proximity of two proteins, as well as the expression levels of individual proteins. Future Science Ltd 2019-12-17 2019-11 /pmc/articles/PMC7092707/ /pubmed/31859535 http://dx.doi.org/10.2144/btn-2019-0083 Text en © 2019 Jonathan A Javitch This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Reports Zhu, Ying Mészáros, József Walle, Roman Fan, Rongxi Sun, Ziyi Dwork, Andrew J Trifilieff, Pierre Javitch, Jonathan A Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier |
title | Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier |
title_full | Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier |
title_fullStr | Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier |
title_full_unstemmed | Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier |
title_short | Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier |
title_sort | detecting g protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a bayesian classifier |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092707/ https://www.ncbi.nlm.nih.gov/pubmed/31859535 http://dx.doi.org/10.2144/btn-2019-0083 |
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