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Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples
Long RT-PCR (LRP) amplification of RNA templates is sometimes difficult compared to long PCR of DNA templates. Among RNA templates, hepatitis C virus (HCV) represents an excellent example to challenge the potential of LRP technology due to its extensive secondary structures and its difficulty to be...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092855/ https://www.ncbi.nlm.nih.gov/pubmed/16793008 http://dx.doi.org/10.1016/j.bbrc.2006.06.039 |