Cargando…

Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples

Long RT-PCR (LRP) amplification of RNA templates is sometimes difficult compared to long PCR of DNA templates. Among RNA templates, hepatitis C virus (HCV) represents an excellent example to challenge the potential of LRP technology due to its extensive secondary structures and its difficulty to be...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fan, Xiaofeng, Xu, Yanjuan, Di Bisceglie, Adrian M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Inc. 2006
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092855/ https://www.ncbi.nlm.nih.gov/pubmed/16793008 http://dx.doi.org/10.1016/j.bbrc.2006.06.039

Ejemplares similares

A method for near full-length amplification and sequencing for six hepatitis C virus genotypes
por: Bull, Rowena A., et al.
Publicado: (2016)

Genome-wide capture sequencing to detect hepatitis C virus at the end of antiviral therapy
por: Peng, Peng, et al.
Publicado: (2020)

Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome
por: Tam, Albert W., et al.
Publicado: (1991)

Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
por: Isaacs, Sonia R., et al.
Publicado: (2018)

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
por: Hiener, Bonnie, et al.
Publicado: (2018)

High-Resolution Quantification of Hepatitis C Virus Genome-Wide Mutation Load and Its Correlation with the Outcome of Peginterferon-Alpha2a and Ribavirin Combination Therapy
por: Wang, Weihua, et al.
Publicado: (2014)

SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones
por: Wellenreuther, Ruth, et al.
Publicado: (2004)

The preparation of an infectious full-length cDNA clone of Saffold virus
por: Himeda, Toshiki, et al.
Publicado: (2011)

Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping
por: Johnston, Camille Melissa, et al.
Publicado: (2018)

A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion
por: Peng, Peng, et al.
Publicado: (2020)

Construction and characterization of a full-length infectious clone of Getah virus in vivo
por: Ren, Tongwei, et al.
Publicado: (2022)

Development of a Full-Length Infectious Cdna Clone of the Grapevine Berry Inner Necrosis Virus
por: Fan, Xudong, et al.
Publicado: (2020)

Identification, Molecular Cloning, and Analysis of Full-Length Hepatitis C Virus Transmitted/Founder Genotypes 1, 3, and 4
por: Stoddard, Mark B., et al.
Publicado: (2015)

Prospects for personalizing antiviral therapy for hepatitis C virus with pharmacogenetics
por: Tavis, John E, et al.
Publicado: (2011)

Single molecule, near full-length genome sequencing of dengue virus
por: Adikari, Thiruni N., et al.
Publicado: (2020)

Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
por: Silva, J.V.J., et al.
Publicado: (2015)

Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis
por: Polyak, Stephen J, et al.
Publicado: (2005)

An efficient full-length cDNA amplification strategy based on bioinformatics technology and multiplexed PCR methods
por: Chen, Nan, et al.
Publicado: (2016)

Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning
por: Tuo, Decai, et al.
Publicado: (2015)

Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
por: Anderson, Motswedi, et al.
Publicado: (2018)

Cloning and Expression of Highly Pathogenic Avian Influenza Virus Full-Length Nonstructural Gene in Pichia pastoris
por: Abubakar, M. B., et al.
Publicado: (2011)

Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
por: Santos, Jefferson José da Silva, et al.
Publicado: (2015)

Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus
por: Tu, Liqin, et al.
Publicado: (2021)

Full coding hepatitis E virus genotype 3 genome amplification method
por: Muñoz-Chimeno, M., et al.
Publicado: (2016)

Construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain
por: Chen, Liu, et al.
Publicado: (2013)

Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones
por: Johansen, I. Elisabeth, et al.
Publicado: (2008)

Recovery of infectious virus from full-length cowpox virus (CPXV) DNA cloned as a bacterial artificial chromosome (BAC)
por: Roth, Swaantje J, et al.
Publicado: (2011)

PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
por: Chen, Yi-Ning, et al.
Publicado: (2015)

A4 An amplicon-based approach for universal amplification, sequencing, and assembly of full-length HIV-1 samples from the DRC
por: Bletsa, M, et al.
Publicado: (2019)

Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg
por: Bordat, Amandine, et al.
Publicado: (2015)

Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone
por: Feng, Mingfeng, et al.
Publicado: (2016)

Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence
por: Li, Jie, et al.
Publicado: (2017)

Bacterial artificial chromosome-based reverse genetics system for cloning and manipulation of the full-length genome of infectious bronchitis virus
por: Inayoshi, Yujin, et al.
Publicado: (2022)

Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses
por: Cheng, Wei-xia, et al.
Publicado: (2010)

Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method
por: Shen, Quan, et al.
Publicado: (2007)

Transgenic Mouse Expressing a Full‐length Hepatitis C Virus cDNA
por: Matsuda, Jun‐ichi, et al.
Publicado: (1998)

Rapid CRISPR/Cas9-Mediated Cloning of Full-Length Epstein-Barr Virus Genomes from Latently Infected Cells
por: Yajima, Misako, et al.
Publicado: (2018)

Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains
por: Zhang, Lizhou, et al.
Publicado: (2018)

Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China
por: Fan, Baochao, et al.
Publicado: (2017)

Functional annotation of 19,841 Populus nigra full-length enriched cDNA clones
por: Nanjo, Tokihiko, et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_feuqum0ammh2v06sgjimqkvfkb