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Can we use it? On the utility of de novo and reference-based assembly of Nanopore data for plant plastome sequencing

The chloroplast genome harbors plenty of valuable information for phylogenetic research. Illumina short-read data is generally used for de novo assembly of whole plastomes. PacBio or Oxford Nanopore long reads are additionally employed in hybrid approaches to enable assembly across the highly simila...

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Detalles Bibliográficos
Autores principales: Scheunert, Agnes, Dorfner, Marco, Lingl, Thomas, Oberprieler, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092973/
https://www.ncbi.nlm.nih.gov/pubmed/32208422
http://dx.doi.org/10.1371/journal.pone.0226234

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