Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9

CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure. However, the binding of DNA sequences that do not fully match the guide RNA can limit...

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Detalles Bibliográficos
Autores principales: Mitchell, Brandon P., Hsu, Rohaine V., Medrano, Marco A., Zewde, Nehemiah T., Narkhede, Yogesh B., Palermo, Giulia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093078/
https://www.ncbi.nlm.nih.gov/pubmed/32258048
http://dx.doi.org/10.3389/fmolb.2020.00039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093078/
https://www.ncbi.nlm.nih.gov/pubmed/32258048
http://dx.doi.org/10.3389/fmolb.2020.00039

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